A 3D Printable microscope and translation stage.
The trick of making a microscope out of a webcam has been around for a little while, and produces good results. However, getting a nice mechanical stage to focus the microscope and move around on the sample is tricky. This project is a 3D printable design that enables very fine (sub-micron) mechanical positioning of the sample and the lens, with surprisingly good mechanical stability. It’s discussed in a paper in Review of Scientific Instruments (open access).
This project is open-source and is released under the CERN open hardware license.
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ABSTRACT
Open source hardware has the potential to revolutionise the way we build scientific instruments; with the advent of readily available 3D printers, mechanical designs can now be shared, improved, and replicated faster and more easily than ever before. However, printed parts are typically plastic and often perform poorly compared to traditionally machined mechanisms. We have overcome many of the limitations of 3D printed mechanisms by exploiting the compliance of the plastic to produce a monolithic 3D printed flexure translation stage, capable of sub-micron-scale motion over a range of 8 × 8 × 4 mm. This requires minimal post-print clean-up and can be automated with readily available stepper motors. The resulting plastic composite structure is very stiff and exhibits remarkably low drift, moving less than 20 μm over the course of a week, without temperature stabilisation. This enables us to construct a miniature microscope with excellent mechanical stability, perfect for time-lapse measurements in situ in an incubator or fume hood. The ease of manufacture lends itself to use in containment facilities where disposability is advantageous and to experiments requiring many microscopes in parallel. High performance mechanisms based on printed flexures need not be limited to microscopy, and we anticipate their use in other devices both within the laboratory and beyond.
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